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Diagnostic Services

CentralStar’s Laboratories provide sample analyses on milk, blood, fecal and tissue samples for a variety of production, disease and health related traits.

More than 4.3 million samples are processed annually using state of the art equipment and techniques including infrared spectroscopy, flow cytometry, ELISA, PCR and more.

Results

Component, SCC and MUN results are reported by email or mail. All other diagnostic testing results are available online, via email and text messaging.

Submitting Samples

Milk samples can be submitted through routine DHI testing or sent direct to the Laboratories. All other sample types should be sent direct to the laboratory closest to you. A completed Sample Submission Form should accompany all samples. For convenience, shippers and sampling supplies can be purchased here.

CentralStar Michigan Lab
1163 Comet Lane, Grand Ledge, MI 48837
517.333.8381 – [email protected]

CentralStar Wisconsin Lab Complex
200 East Kelso Road, Kaukauna, WI  54130
920.733.6598 – [email protected]

Pregnancy 

Sample Type(s)

  • Milk (fresh, frozen or preserved)
  • Blood

Timing

  • Test as early as 28 days post-breeding through dry-off
  • Must be at least 60 days post-calving for milk ELISA

Results

  • Available within 24 hours of sample processing
  • Reported as Pregnant, Open, or Recheck based on the level of the PAGs present
  • Cows with a Recheck result should be verified by veterinarian or retested

Accuracy

  • 98% accurate; comparable to palpation and ultrasound

Price

  • Milk $4.50/sample
  • Blood $3.75/sample
  • Multiple test discount! $10 for two ELISAs on same sample; $13.50 for 3 ELISAs
  • Testing billed upon sample receipt

Submit Samples

Mastitis PCR

  • PCR detection effective on DHI or hand-stripped milk samples from treated, fresh or late lactation cows
  • Pool up to five samples for cost-effective screening. Split positive pools to test individual samples
  • Convenient testing strategies:
    • High SCC samples focus on cows most likely to be positive
    • Fresh cows to optimize detection and minimize herd impact
    • Hospital pen to monitor infection status and verify clearance before reintroduction
    • Bulk tank or groups to provide ongoing surveillance

Sample Type(s)

  • Milk (fresh, frozen or preserved)

Results

  • Available within 48 hours of sample processing
  • Only organisms detected are indicated. If no organisms are detected, result is reported as Undetected with a Negative final result.
  • Results are reported as Negative or Positive. Positive results are given a categorical assessment of + (low), ++ (intermediate), or +++ (high) based on CT values. 

Price

  • Contagious 3: $27/test
  • Complete 16: $35/test
  • Pooling: add $1.00/sample
  • Testing billed upon sample receipt

Submit Samples

Additional Information

Johne’s Disease

  • Johne’s causes decreased milk production, chronic diarrhea, and death.

Sample Type(s)

  • Milk
    • Fresh, frozen or preserved
    • Individual or bulk tank
    • Pooling available for PCR test
  • Blood
    • Fresh
  • Feces
    • Fresh or frozen. Pooling available – up to 5 samples per test

Timing

  • Test milk or blood at dryoff or in early lactation
  • Test fecal samples at any age

Results

Price

  • Individual ELISA (milk, blood) $6/sample
    • Multiple test discount! $10 for two ELISAs on same sample; $13.50 for 3 ELISAs
  • PCR (milk, pooled or bulk milk, fecal, pooled fecal) $40/test
  • Sample pooling add $1.00 per sample
  • Testing billed upon sample receipt

Submit Samples

Additional Resources

Bovine Viral Diarrhea (BVD)

  • Infection reduces reproductive performance by decreasing conception and inducing abortion
  • Persistently infected (PI) calves developed in-utero lead to frequent death, poor performance, and ongoing reinfection within the herd
  • Utilize ELISA (antigen) or PCR analyses and strategic pooling to reduce testing costs

Sample Type(s)

  • Milk
    • Individual (fresh, frozen, or preserved)
    • Bulk tank (fresh or preserved)
  • Blood (fresh)
  • Tissue (fresh or frozen)

Timing

  • Test tissue or blood samples from calves to identify PI animals
  • Test individual or pooled milk samples from cows to efficiently clear adult herd of PI suspicion

Results

Price

  • Individual ELISA (milk, tissue, blood) $6/sample
    • Multiple test discount! $10 for two ELISAs on same sample; $13.50 for 3 ELISAs
  • Group ELISA (pooled tissue) $18/test
    • Pool up to 10 tissues samples
  • Group PCR (pooled tissue, milk) $40/test
    • Pool up to 20 tissue samples or 250 milk samples
  • Sample pooling add $1.00/sample
  • Testing billed upon sample receipt

Submit Samples

Bovine Leukosis (BLV)

  • Blood-borne viral infection with no effective vaccine
  • Negatively impacts production, longevity and cull value
  • Utilize ELISA analyses and strategic pooling to reduce testing costs

Sample Type(s)

  • Milk, individual or bulk tank (fresh, frozen or preserved)
  • Blood (fresh)

Timing

  • Test blood samples on animals over 4 months
  • Determine herd prevalence by testing 10 cows each from four lactations

Results

Price

  • Individual ELISA (milk, blood) $6/sample
    • Multiple test discount! $10 for two ELISAs on same sample; $13.50 for 3 ELISAs
  • Group ELISA (pooled or bulk milk) $18/test
    • Sample pooling add $1.00/sample
  • Testing billed upon sample receipt

Submit Samples

Additional Information

Progesterone

Sample Type(s)

  • Blood
    • Fresh
  • Milk
    • Hand-stripped sample for most accurate results
    • Samples should be chilled or frozen soon after collection, and shipping expedited to optimize detection

Timing

  • Test at time of prostaglandin shot and breeding to verify shot compliance

Results

Price

  • Individual ELISA (milk, blood) $6/sample
    • Multiple test discount! $10 for two ELISAs on same sample; $13.50 for 3 ELISAs
  • Testing billed upon sample receipt

Submit Samples

Additional Information

Neospora

  • Abortions associated with Neospora occur mid-gestation. Dogs are definitive hosts, capable of shedding oocysts in feces after eating infected tissue. Oocysts are able to survive in soil and water beyond the decomposition of the feces. Cattle become infected by ingesting oocysts.
  • Infection can also pass vertically from dam to fetus in cattle

Sample Type(s)

  • Milk (fresh, frozen or preserved)
  • Blood (fresh)

Timing

  • Test individuals or groups of aborting and non-aborting cows to associate Neospora with abortions

Results

Price

  • Individual ELISA (milk, blood) $6/sample
  • Multiple test discount! $10 for two ELISAs on same sample; $13.50 for 3 ELISAs
  • Testing billed upon sample receipt

Submit Samples

Additional Information

Non-Esterified Fatty Acid (NEFA)

  • Healthy animals typically have low NEFA concentrations in the blood
  • Animals in the transition period (3 weeks pre- to 3 weeks post-calving) may experience high NEFA concentrations due to increased energy requirements.
  • Metabolism of blood NEFA can result in fatty liver, ketosis and inflammation.
  • Animals experiencing high NEFA concentrations are also at greater risk for displaced abomasum, metritis, retained fetal membranes, lameness and even prolonged return to estrus.

Sample Type(s)

  • Blood
    • Chill samples as soon as possible after collection and expedite shipping to optimize detection

Timing

  • Test 15 cows 0-14 days pre and/or post-calving to effectively evaluate transition management program

Results

Price

  • Individual ELISA $15/sample
  • Testing billed upon sample receipt

Submit Samples

SCC, Components, MUN

Sample Type

  • Milk (preserved)

Sample Handling

  • Keep out of direct sunlight or heat
  • Invert one hour after taking sample to mix preservative
  • Ship within 24-48 hours of collection

Results

  • SCC & Components within 24 hours of sample processing, MUN 3 days.
  • Emailed in CSV or Excel format, and mailed

Price

  • SCC/Components $1/sample
  • MUN $1/sample
  • MUN/SCC/Components $2/sample
  • Submission Fee $5/order
  • Testing billed upon sample receipt

Submit Samples

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