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milk vials for mastitis testing

Mastitis PCR

  • PCR detection effective on DHI or hand-stripped milk samples from treated, fresh or late lactation cows
  • Pool up to five samples for cost-effective screening. Split positive pools to test individual samples
  • Convenient testing options include:
    • High SCC samples
    • Fresh cows
    • Monitor causes of infection and verify clearance before introduction
    • Bulk tank or groups to provide ongoing surveillance

PCR Test Options:

  • Individual Organism PCR
    • Staphyloccous aureus or Mycoplasma bovis 
    • Staphylococcus aureus is one of the most common pathogens to cause chronic mastitis. It is highly contagious and usually not treatable.  Infection with Staph aureus often leads to abscesses and an abundance of scar tissue in the mammary gland.
    • Mycoplasma bovis is a very small, highly contagious bacteria that can cause mastitis as well as respiratory and reproductive issues. Infection with Mycoplasma bovis is not treatable; infected cows must be identified, segregated, and culled.
  • Contagious 4 PCR
    • M bovis, Strep ag., Staph aureus, Strep uberis
  • Complete 16 PCR
    • Staphylococcus aureus, Staphylococcus species, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Escherichia coli, Corynebacterium bovis, Enterococcus faecalis , & faecium, Klebsiella pneumoniae & oxytoca, Serratia marcescens, Arcanobacterium pyogenes & Peptostreptococcus indolicus, Staphylococcal beta-lactamase gene, Mycoplasma bovis, Mycoplasma species, Yeast and Prototheca species.

Sample Type(s)

  • Milk (fresh, frozen or preserved)


  • Available within 48 hours of sample processing
  • Only organisms detected are indicated. If no organisms are detected, result is reported as Undetected with a Negative final result.
  • Results are reported as Negative or Positive. Positive results are given a categorical assessment of + (low), ++ (intermediate), or +++ (high) based on CT values


  • Complete 16: $40
  • Pooled Complete 16 (Min 3/ Max 5): $10
  • Contagious 4: $30
  • Pooled Contagious 4 (Min 3/Max 5): $8
  • Single Mastitis (Staph aureus OR M. bovis): $14.25
  • Pooled Single Mastitis (Min 3/ Max 5): $4
  • Testing billed upon sample receipt
  • $10 sample submission fee per order

Submit Samples

Additional Information

PCR mastitis testing for herd health and milk quality

CentralStar Laboratories

CentralStar’s Laboratories provide sample analyses on milk, blood, fecal, and tissue samples for a variety of production, disease and health-related traits.

More than 5.3 million samples are processed annually using state-of-the-art equipment and techniques including infrared spectroscopy, flow cytometry, ELISA, PCR, and more.

Testing Supplies

For convenience, shippers and sampling supplies can be purchased online.

Diagnostic tests are intended to identify diseases in the cow for their health, and do not determine the “safety” of milk. Testing prices listed effective 10/1/2020. 

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