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Diagnostic Services

CentralStar’s Laboratories provide sample analyses on milk, blood, fecal, and tissue samples for a variety of production, disease and health-related traits. milk-based cow pregnancy test kit dairy cow milk pregnancy test Milk Pregnancy testing, cow pregnancy testing mil0k

More than 5.3 million samples are processed annually using state-of-the-art equipment and techniques including infrared spectroscopy, flow cytometry, ELISA, PCR, and more. dairy cow milk pregnancy test Milk Pregnancy testing, cow pregnancy testing milk

Results

Component, SCC, and MUN results are reported by email or mail. All other diagnostic testing results are available online, via email, and text messaging.

Submitting Samples

Milk samples can be submitted through routine DHI testing or sent directly to the Laboratories. All other sample types should be sent directly to the laboratory closest to you. A completed Sample Submission Form should accompany all samples. For convenience, shippers and sampling supplies can be purchased here. Milk Pregnancy testing, cow pregnancy testing milk

When submitting blood samples use red-top tubes for serum and purple-top tubes for whole blood. Lab services

View these guidelines for answers to general questions on sampling and shipping.

CentralStar Michigan Lab
1163 Comet Lane – Suite 100, Grand Ledge, MI 48837
517.333.8381 – [email protected]

CentralStar Wisconsin Lab Complex
200 East Kelso Road, Kaukauna, WI  54130
920.733.6598 – [email protected]

Pregnancy 

Sample Type(s)

Timing

  • Test as early as 28 days post-breeding through dry-off
  • Must be at least 60 days post-calving for milk ELISA

Results

  • Available within 24 hours of sample processing
  • Reported as Pregnant, Open, or Recheck based on the level of the PAGs present
  • Cows with a Recheck result should be verified by veterinarian or retested

Accuracy

  • 98% accurate; comparable to palpation and ultrasound

Price

  • Milk $4.75/sample
  • Serum $4.00/sample
  • Multiple test discount! $11 for two ELISAs on same sample; $15.00 for 3 ELISAs
  • Testing billed upon sample receipt

Submit Samples

Mastitis PCR

  • PCR detection effective on DHI or hand-stripped milk samples from treated, fresh or late lactation cows
  • Pool up to five samples for cost-effective screening. Split positive pools to test individual samples
  • Convenient testing options include:
    • High SCC samples
    • Fresh cows 
    • Hospital pen to monitor infection status and verify clearance before introduction
    • Bulk tank or groups to provide ongoing surveillance

PCR Test Options:

  • Individual Organism PCR
    • Staphyloccous aureus or Mycoplasma bovis 
  • Contagious 4 PCR
    • M bovis, Strep ag., Staph aureus, Strep uberis
  • Complete 16 PCR
    • Staphylococcus aureus, Staphylococcus species, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Escherichia coli, Corynebacterium bovis), Enterococcus faecalis , & faecium, Klebsiella pneumoniae & oxytoca, Serratia marcescens, Arcanobacterium pyogenes & Peptostreptococcus indolicus, Staphylococcal beta-lactamase gene, Mycoplasma bovis, Mycoplasma species, Yeast and Prototheca species.

Sample Type(s)

  • Milk (fresh, frozen or preserved)

Results

  • Available within 48 hours of sample processing
  • Only organisms detected are indicated. If no organisms are detected, result is reported as Undetected with a Negative final result.
  • Results are reported as Negative or Positive. Positive results are given a categorical assessment of + (low), ++ (intermediate), or +++ (high) based on CT values. 

Price

  • Complete 16: $40
  • Pooled Complete 16 (Min 3/ Max 5): $10
  • Contagious 4: $30
  • Pooled Contagious 4 (Min 3/Max 5): $8
  • Single Mastitis (Staph aureus OR M. bovis): $12
  • Pooled Single Mastitis (Min 3/ Max 5): $4
  • Testing billed upon sample receipt

Submit Samples

Additional Information

Staph aureus Mastitis PCR

  • Detects Staphylococcus aureus in infected milk.
  • Staphylococcus aureus is one of the most common pathogens to cause chronic mastitis. It is highly contagious and usually not treatable.  Infection with Staph aureus often leads to abscesses and an abundance of scar tissue in the mammary gland.

Mycoplasma bovis Mastitis PCR

  • Detects Mycoplasma bovis in infected milk.
  • Mycoplasma bovis is a very small, highly contagious bacteria that can cause mastitis as well as respiratory and reproductive issues. Infection with Mycoplasma bovis is not treatable; infected cows must be identified, segregated, and culled.

Johne’s Disease

  • Johne’s causes decreased milk production, chronic diarrhea, and death.

Sample Type(s)

  • Milk
    • Fresh, frozen or preserved
    • Individual or bulk tank
    • Pooling available for PCR test
  • Serum
    • Fresh
  • Feces
    • Fresh or frozen. Pooling available – up to 5 samples per test

Timing

  • Test milk or blood at dryoff or in early lactation
  • Test fecal samples at any age

Results

Price

  • Individual ELISA (milk, blood) $6.50/sample
    • Multiple test discounts! $11 for two ELISAs on the same sample; $15.00 for 3 ELISAs
  • PCR (milk, pooled, or bulk milk) $50/test
  • PCR (fecal) $40/test
  • PCR (pooled fecal) $12/test; minimum 3 samples, maximum 5 samples
  • Testing billed upon sample receipt

Submit Samples

Additional Resources

Bovine Viral Diarrhea (BVD)

  • Infection reduces reproductive performance by decreasing conception and inducing abortion
  • Persistently infected (PI) calves developed in-utero lead to frequent death, poor performance, and ongoing reinfection within the herd
  • Utilize ELISA (antigen) or PCR analyses and strategic pooling to reduce testing costs
  • More information

Sample Type(s)

  • Milk
    • Individual (fresh, frozen, or preserved)
    • Bulk tank (fresh or preserved)
  • Serum (fresh)
  • Tissue (fresh or frozen)

Timing

  • Test tissue or blood samples from calves to identify PI animals
  • Test individual or pooled milk samples from cows to efficiently clear adult herd of PI suspicion

Results

Price

  • Individual ELISA (milk, tissue, blood) $6.50/sample
    • Multiple test discount! $11 for two ELISAs on same sample; $15.00 for 3 ELISAs
  • Individual PCR (milk, tissue, whole blood) $40
  • Group PCR (pooled tissue, milk) $3.70/sample
    • Pool up to 20 tissue samples and up to 250 milk samples
  • Testing billed upon sample receipt

Submit Samples

Bovine Leukosis (BLV)

  • Blood-borne viral infection with no effective vaccine
  • Negatively impacts production, longevity and cull value
  • Utilize ELISA analyses and strategic pooling to reduce testing costs
  • Producers can screen cows by ELISA then submit samples from BLV-positive cows for PCR testing. 
    • The PCR test can identify susceptible cows that have become “Super Shedders,” causing the most risk to uninfected herdmates and youngstock.

Sample Type(s)

  • Milk, individual or bulk tank (fresh, frozen or preserved)
  • Serum (ELISA)
  • Whole Blood (PCR or ELISA)

Timing

  • Test milk at dry-off or fresh
  • Herd prevalence can be estimated by testing 10 cows each from lactations 1, 2, 3, and 4+ at DHI test
  • Test blood by ELISA on animals over 4 months
  • Test blood by PCR at any age

Results

  • Available within 5 business days of sample receipt
  • PCR results available within 10 business days of sample receipt
    • Reported as High, Moderate, Low, or Undetected with a numeric value indicating the number of BLV copies per white blood cell.

Price

  • Individual ELISA (milk, blood) $6.50/sample
    • Multiple test discount! $11for two ELISAs on same sample; $15.00 for 3 ELISAs
  • Individual PCR $12/test
  • Group ELISA (pooled or bulk milk) $20/test
  • Testing billed upon sample receipt

Submit Samples

Additional Information

Neospora

  • Abortions associated with Neospora occur mid-gestation. Dogs are definitive hosts, capable of shedding oocysts in feces after eating infected tissue. Oocysts are able to survive in soil and water beyond the decomposition of the feces. Cattle become infected by ingesting oocysts.
  • Infection can also pass vertically from dam to fetus in cattle

Sample Type(s)

  • Milk (fresh, frozen or preserved)
  • Serum (fresh)

Timing

  • Test individuals or groups of aborting and non-aborting cows to associate Neospora with abortions

Results

Price

  • Individual ELISA (milk, blood) $6.50/sample
  • Multiple test discount! $11 for two ELISAs on same sample; $15.00 for 3 ELISAs
  • Testing billed upon sample receipt

Submit Samples

Additional Information

A1/A2 (beta-casein) 

The A2 genotyping assay identifies the A1 and A2 beta-casein status of individual animals, providing insight for strategic breeding to develop A2 herd status. The A2 isoform of beta-casein is potentially better digested that the A1 isoform. Milk containing only the A2 isoform (A2/A2) is currently being marketed as an alternative to traditional fluid milk.

Sample Type(s)

  • Whole Blood 
  • Tissue (fresh or frozen)
  • Milk (fresh, frozen, or preserved) 

Timing

  • Test at any age or time at your convenience

Results

  • Available within 10 days of sample receipt
  • Reported as A1/A1, A1/A2, A2/A2, or retest

Accuracy

  • Blood and tissue: 99% accurate
  • Milk: 94% accurate; ‘Retests’ should be verified by blood or tissue

Price

  • $12/sample 
  • Testing billed upon sample receipt

Submit Samples

SCC, Components, MUN

Sample Type

  • Milk (preserved)

Sample Handling

  • Keep out of direct sunlight or heat
  • Invert one hour after taking sample to mix preservative
  • Ship within 24-48 hours of collection

Results

  • SCC & Components within 24 hours of sample processing, MUN 3 days.
  • Emailed in CSV or Excel format, and mailed

Price

  • SCC/Components $1.50/sample
  • MUN $1.50/sample
  • MUN/SCC/Components $3/sample
  • Submission Fee $10/order
  • Testing billed upon sample receipt

Submit Samples

Diagnostic tests are intended to identify diseases in the cow for their health, and do not determine the “safety” of milk. Testing prices listed effective 10/1/2020. 
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